rna seq transcriptome analysis  (Gene Co Ltd)

Journal: Microbial Biotechnology

Article Title: Regulation of Sugar Metabolism During Fermentation of Brewers' Spent Grain by Leuconostoc pseudomesenteroides DSM20193

doi: 10.1111/1751-7915.70116

Figure Lengend Snippet: Overview of the transcriptome analysis outcomes highlighting the carbohydrate metabolism relevant for the BSG fermentation. Analysis of differential gene expression between 24‐h MRS cultivation (equivalent to T0 of the BSG fermentation) and 16‐h ESP + BSG fermentation. (A) The box plot shows the distribution of gene expression levels between BSG and MRS samples (the boxes represent the interquartile range (IQR), the line inside each box shows the median, whiskers extend to 1.5 times the IQR, dots represent outliers, and ‘x’ marks indicate the mean values). (B) The bar chart showing the number of differentially expressed genes between BSG and MRS samples. (C) The dot plot illustrating the overall level of expression of different functional groups of genes involved in carbohydrate metabolism under BSG and MRS conditions (1: maltose phosphorylases, 2: sucrose hydrolases and phosphorylases and fructose bisphosphatases, 3: dTDP‐glucose, phosphoketolase, galactokinase, and G6PDH, 4: sucrose, maltose hydrolases and UTP/UDP‐glucose enzymes, 5: lipopolysacchardie and peptidoglycan biosynthesis, 6: glycosyltransferase family proteins, 7: xylose isomerases and arabinose transporters, 8: isomerases and mutarotase enzymes: glucose, galactose, and phosphate transfer, and 9: PTS system components). (D) The bar chart depicts the fold changes in expression (ESP + BSG T16 vs. MRS 24 h ≈ BSG T0) of selected individual genes encoding the enzymes relevant to the metabolism of carbohydrates as monitored in the BSG fermentations.

Article Snippet: The statistical analysis for the transcriptome analysis (RNA‐seq) was performed at Novogene (Table ).

Techniques: Gene Expression, Expressing, Functional Assay